Genetic Transformation of an argB Mutant of Aspergillus oryzae.
نویسندگان
چکیده
An argB mutant of Aspergillus oryzae NRRL 492 has been genetically transformed with the Aspergillus nidulans argB gene. Protoplasts were generated with a combination of Novozyme 234 and beta-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for A. nidulans. A frequency of 5 to 10 transformants per mug of DNA was obtained; however, most transformants appeared abortive. The A. nidulans argB gene and vector sequences appeared to be integrated into the A. oryzae chromosome.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 54 6 شماره
صفحات -
تاریخ انتشار 1988